INTEGRA’s ASSIST PLUS pipetting robot helps streamline sample pooling for arbovirus testing

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Lab supervisor Denise Bolton explained: “We adopted pooled testing in the summer of 2020, initially building the sample pools manually. However, the extra step complicated the workflow, required high levels of focus and concentration, and took up more than an hour of analyst time. Adding a manual pooling step was also quite risky from a quality standpoint, and we were concerned about the risk of analyst error. »

“We were already familiar with the ASSIST PLUS system, as we use it for our COVID-19 samples. It was easy to write a pooling program using VIALAB software, and the flexibility of the VOYAGER 8-channel adjustable tip spacing pipette meant that there was no need to change our processing method. mosquitoes. We use the platform to transfer aliquots of 2ml tubes into a 96-well plate to create the sample pools, then add the lysis buffer directly, using the entire pool for acid extraction nucleic. This streamlines the process, saves time, and reduces the use of plastic labware, since we are not building high volume pools and then transferring an aliquot for extraction. We saw a 75% saving on extraction and PCR reagents, and reduced PCR instrument usage for the monitoring program, as well as saving valuable time for analysts. More importantly, we are confident that the most complex step of the process is executed error-free. »
INTEGRA’s ASSIST PLUS pipetting robot helps streamline sample pooling for arbovirus testing


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